A simple and specific HPTLC method has been developed for simultaneous estimation of Montelukast Sodium and Olopatadine Hydrochloride in bulk and simulated dosage form. The method employed HPTLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. Methanol: Toluene: Ethyl acetate: Ammonia in ratio of 4.6: 4: 1: 0.4 v/v/v/v is used as a mobile phase. The Rf value of Montelukast Sodium and Olopatadine Hydrochloride were found 0.81±0.0081 and 0.20±0.0080 respectively. The linearity for Montelukast Sodium and Olopatadine Hydrochloride were found to be in range of 400-2400 ng/band and 200-1200 ng/band respectively. The % recoveries of Montelukast Sodium and Olopatadine Hydrochloride were found to be in the range of 98.12-99.78% and 98.96-101.76% respectively. The proposed method was validated and successfully applied for the estimation of Montelukast Sodium and Olopatadine Hydrochloride in simulated dosage form.
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